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. 2013 Aug 5;8(8):e70927. doi: 10.1371/journal.pone.0070927

Figure 1. Microglia potentiate neuron death following in vitro ischemia.

Figure 1

Representative images of mixed neuron astrocyte (NA) cultures prior to oxygen glucose deprivation (OGD) (A, NA con) and after (B, NA OGD). Primary microglial cells added to NA cultures are visible as small phase bright cells (C. NAM con). Following OGD, the previously confluent NA cell layer is disrupted leaving mostly microglial cells which tend to form clusters (D, NAM OGD). E: Mixed cultures of primary neurons and astrocytes (NA) were prepared and either primary microglia (NAM) or BV2 cells were added (NAB). Neuron death due to OGD was increased in mixed cultures containing either primary microglia (NAM) or BV2 cells (NAB), and the extent of death was similar. Microglia appear to require cell-cell contact, since BV2 cells did not potentiate neurotoxicity when cultured in inserts (NABi). (*P<0.001 vs. NA, NABi).