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. 2004 Feb 11;32(3):1103–1112. doi: 10.1093/nar/gkh236

Figure 2.

Figure 2

Figure 2

Determination of the stoichiometry between T antigen and topo I in complex with origin DNA. Purified T antigen was incubated with an origin DNA fragment in the presence of different amounts of purifed topo I as shown. After cross-linking, the complexes were subjected to gel electrophoresis followed by western blotting. (A) T antigen blot showing the positions on the gel of single (TH) and double (TDH) hexamers. At the bottom are dot blots with known amounts of T antigen. (B) Topo I blot of the same gel. The position of double hexamers is shown. Topo I dot blots with known amounts of protein are shown at the bottom. (C) Plots of signal strength versus amounts of T antigen or topo I in dot blots. The long arrows correspond to the amounts of T antigen or topo I present at 60 or 70 ng topo I in the reaction.