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Effect of RPA on assembly of complex. (A) Biotinylated origin DNA (–64 to 128) was incubated with 450 ng T antigen, 40 ng topo I, 91 ng pol/prim and various amounts of RPA as shown. The relative T antigen, topo I and RPA signals are plotted, as is the relative topo I to T antigen ratio (arbitrarily set at 1 in the absence of RPA). (B) Same experiment except that pol/prim was missing.
