Figure 6.

Comparison of siRNA-based RNAi and DNA-based RNAi in HeLa cells. The predicted sequences of hairpin-type transcripts are shown on the left, while induced RNAi activity (reduction in relative luciferase activity) is shown by open boxes on the right. Stippled boxes indicate relative luciferase activity reduction due to cognate siRNA in HeLa cells. On the left, predicted AS are shaded. Note that the sequence preference of DNA-based RNAi is essentially identical to that of siRNA-based RNAi. Data obtained from 2–4 experiments were averaged and are shown.