Table 1.
Detection of recombinant SsTPx in infected S. scabiei serum, negative serum and controls by dot-ELISA
| Groups (n = 203) |
Observer 1 number |
Sensitivity or specificity |
Observer 2 number |
Sensitivity or specificity | Kappa index* | Proportion agreement(PA) | ||
|---|---|---|---|---|---|---|---|---|
| + | – | + | – | |||||
|
S. scabiei infected-(n=85) |
81 |
4 |
95.3% |
80 |
5 |
94.1% |
0.882 |
98% |
| Total uninfected (n=118) |
7 |
111 |
94.1% |
8 |
110 |
93.2% |
0.928 |
99% |
| Negative (n=94) |
0 |
94 |
100% |
0 |
94 |
100% |
1.0 |
100% |
|
E.stiedae (n=6) |
0 |
6 |
100% |
0 |
6 |
100% |
1.0 |
100% |
|
T.pisiformis (n=6) |
0 |
6 |
100% |
0 |
6 |
100% |
1.0 |
100% |
| P. cuniculi (n=12) | 7 | 5 | 68.3% | 8 | 4 | 66.7% | 0.823 | 91% |
*Strength of agreement: K index <0, poor; 0–0.20, slight; 0.21–0.4, fair; 0.41–0.6, moderate; 0.61–0.80, substantial; 0.81–1.0; almost perfect.