Table 1. Primers and PCR conditions used for the amplification of coding exons of MYOC and CYP1B1.
| Fragment | Primer | Sequence (5’-3’) | Annealing temperature | Amplicon size (bp) |
|---|---|---|---|---|
|
MYOC-1 |
MYOC-1F |
CTCTGGAGCTCGGGCATG |
56.9 °C |
753 |
| MYOC-1R |
TCACTACGAGCCATATCACC |
|||
|
MYOC-2 |
MYOC-2F |
CATAGTCAATCCTTGGGC |
54.5 °C |
380 |
| MYOC-2R |
CTGCAGACCTGCTCTGACAA |
|||
|
MYOC-3A
MYOC-3B |
MYOC-3AF |
GATTTGTCTCCAGGGCTGTC |
58.4 °C |
724 |
| MYOC-3AR |
CTGCTGAGGTGTAGCTGCTG |
|||
| MYOC-3BF |
TCTGTGGCACCTTGTACACC |
56 °C |
994 |
|
| MYOC-3BR |
ACATCTCCCAACCTGAAGGAA |
|||
|
CYP1B1–2 |
CYP1B1–3F |
GGCGCCCGCTCCTGTCTCTG |
61.4 °C |
1216 |
| CYP1B1–3R |
ACCTGGAGCGAAACCCCAAA |
|||
| CYP1B1–3 | CYP1B1–4F |
AATGTGCTTTCTAGATGAAATAAGAA |
54.6 °C |
751 |
| CYP1B1–4R | CAGCACAAAAGAGGAACTGGA |