Table 1. Primers used for mutation analysis in normal controls by allele-specific PCR.
| Mutation | Primer sequence (5′-3′) | Tm (°C) | Amplicon size (bp) |
|---|---|---|---|
| c.1831T>C |
F:GCGTGCGAGAGATCCTGTCCGACC
14R*: AGTAGGGGACAGGCTACTGCTATGCC |
70 |
168 |
| c.1249G>A |
F:GACCATAGCCGGGCAGAGCATCA
11R*:GGGCTGAACCAGATCCCAAGCCTTGA |
66 |
379 |
| c.2170C>G |
F:CTTGGATCCATGCCGCCTACCCCGA
16R*:GGCCAGAGGCTCCCCACTCCTCAG |
61 |
149 |
| c.785C>T | 8F*:CCCGGGCAGGGCCTCCTCTGTTTC R:GCGCGCCACCTCCATCGCAGTCTTAA | 72 | 86 |
Abbreviations: F, forward primer; R, reverse primer, Tm, annealing temperature; and, bp, base pairs. *Primers are described in Kumar et al. [12].