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. 2013 Aug 5;8(8):e70891. doi: 10.1371/journal.pone.0070891

Figure 2. Selective activation of Akt in AML-M5 cells.

Figure 2

A, Selective activation of Akt kinase in AML-M5 cells. Level of activated kinases in five AML-M5 (indicated by solid color bars) and three non-AML-M5 (HL-60, U937 and K562- indicated by patterned black and white bars) derived cell lines were determined by screening the human phospho-kinase antibody array (Proteome Profiler™) with whole cell extract of each cell mentioned above. Activity of each kinase available in the array, in these two subsets of cells was quantified by densitometric analysis of all available kinases in the human phospho-kinase array. Relative pixel densities are represented as bar graphs. Results are averages of 3 independent experiments. Only Akt kinase activity, as indicated by the phosphorylation of the Ser473 residue, is selectively upregulated in all N-CoR null AML-M5 derived cell lines as marked by a box. B, Level of activated Akt (pAkt-Ser473) in various AML-M5 and non-AML-M5 derived cell lines were determined by western blotting. C, Level of activated Akt (pAkt-Ser473) and N-CoR protein in six human AML-M5 patient samples were determined by western blotting.