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. 2013 Aug 5;8(8):e71837. doi: 10.1371/journal.pone.0071837

Figure 4. Assembly of mitochondrial outer membrane proteins into Mmm1 Cys to Ser mutants.

Figure 4

A. Import and assembly of Tom40 was assayed by incubating radiolabeled Tom40 precursor with isolated mitochondria for the indicated times. After import, the mitochondria were reisolated and dissolved in 1% digitonin. The samples were subjected to BNGE, transferred to PVDF membrane and analyzed by autoradiography. The positions of the mature TOM complex, intermediates I and II, and an unknown band (*) are indicated. The time (min) of each import reaction is indicated above the lanes. B. As in A, except radiolabelled porin precursor was imported. The molecular masses of the major complexes containing radiolabeled porin are indicated. C. As in A, but radiolabeled Tom22 precursor was imported into isolated mitochondria from the indicated strains. Mature TOM complex and monomeric Tom22 (M) are indicated. D. Assembly of Tom22 into mitochondria from the indicated strains as in panel C.