Table 2.
Aminoacid substitutions in FabI of USA300 strains passaged either in the absence (C1 and C2) or presence (A1 through A7) of triclosan compared to the genome sequence (34)
| Aminoacid number (from gene start) | C1 | C2 | A1 | A2 | A3 | A4 | A5 | A6 | A7 |
|---|---|---|---|---|---|---|---|---|---|
|
Upstream of fabI | |||||||||
| −74 |
|
|
|
|
|
|
|
M → R |
|
| −32 |
|
|
|
|
|
|
I → T |
|
|
| −19 |
|
|
|
|
|
|
|
|
V → D |
|
fabI | |||||||||
| 24 |
|
|
A → T |
|
|
|
|
|
|
| 101 |
|
|
|
|
|
|
|
D → Y |
|
| 193 |
|
|
|
I → F |
I → F |
|
|
|
|
| 204 | F → L | F → S | F → C | F → C | |||||
Minus positions are upstream of the protein start. Abbreviations for amino acids are: M Methionine, R Arginine, I Isoleucine, T Threonine, V Valine, D aspartic acid, A alanine, N asparagine, F Phenyl alanine, C cysteine, L Leucine, S Serine.