Table 1.
Summary of immune gene expression in T. bryosalmonae infected rainbow trout kidney tissue
| Gene | Grade 0 average cCq |
Kidney swelling grade |
||||
|---|---|---|---|---|---|---|
| Grade 1 | Grade 1-2 | Grade 2 | Grade 3 | Pearson correlation | ||
|
T. bryosalmonae 18S rDNA |
Not detectable |
2105.69** |
4266.94** |
6652.34** |
8034.26** |
.755** |
|
T. bryosalmonae RPL18 |
|
8013.47** |
37233.66** |
39385.81** |
26654.46** |
.740** |
|
IL-1β-1 |
27.16 |
3.71* |
2.24 |
2.10 |
2.31 |
-.116 |
|
TNF-α1 |
24.52 |
1.12 |
0.95 |
0.94 |
0.84 |
-.155 |
|
TNF-α2 |
26.60 |
0.97 |
2.19* |
1.52 |
1.64 |
.106 |
|
IL-6 |
34.12 |
7.44** |
31.53** |
23.59** |
15.38** |
.510** |
|
IL-11 |
29.62 |
31.74** |
25.04** |
14.13** |
11.54** |
.399** |
|
M17 |
26.05 |
5.10** |
6.28** |
5.48** |
2.60 |
.179 |
|
COX-2A |
24.69 |
2.15 |
1.68 |
1.75 |
1.07 |
-.161 |
|
COX-2B |
32.73 |
3.59 |
3.94* |
3.10* |
2.03 |
.164 |
|
MCSF-1 |
22.92 |
1.18 |
0.77 |
0.49* |
0.23** |
-.658** |
|
MCSF-2 |
21.78 |
1.81 |
3.82* |
2.77 |
1.41 |
-.076 |
|
MCSF-R1 |
27.65 |
1.05 |
2.48 |
1.01 |
0.57* |
-.375* |
|
MCSF-R2 |
21.77 |
2.15 |
2.70 |
2.65 |
1.22 |
-.001 |
|
iNOS |
33.31 |
2.34 |
5.54* |
2.43 |
1.28 |
-.035 |
|
Arginase-1 |
15.20 |
1.05 |
0.50* |
0.45* |
0.23** |
-.612** |
|
Cathelicidin-1 |
28.63 |
52.60** |
136.21** |
72.80** |
57.81** |
.530** |
|
Cathelicidin-2 |
29.58 |
9.00** |
56.40** |
94.90** |
19.10** |
.547** |
|
Hepcidin-1 |
25.76 |
5.36* |
10.85* |
22.37** |
6.42** |
.360* |
|
LEAP-2A |
34.31 |
10.92** |
12.87** |
7.76** |
6.84** |
.281 |
|
CD8α |
25.31 |
5.37** |
4.01** |
5.46** |
3.91* |
.238 |
|
CD8β |
21.41 |
2.99* |
3.05* |
3.34* |
2.33 |
.128 |
|
IL-2Rβ |
24.63 |
3.79** |
5.19** |
1.28 |
0.93 |
-.168 |
|
IgD H-secretory |
21.39 |
0.98 |
1.02 |
0.89 |
0.92 |
-.095 |
|
IgM H-secretory |
14.14 |
5.39* |
3.38 |
8.80** |
4.80** |
.399** |
|
IgT H-secretory |
21.31 |
8.19* |
24.68** |
51.03** |
68.62** |
.732** |
|
CD4 |
22.47 |
3.66** |
4.28** |
4.72** |
3.56* |
.298 |
|
T-bet |
24.94 |
7.00** |
6.86** |
7.06** |
2.80** |
.203 |
|
IL-2 |
28.68 |
3.68* |
4.16** |
2.16 |
2.54 |
.048 |
|
IFNγ |
29.25 |
5.06** |
15.40** |
10.03** |
8.49** |
.500** |
|
GATA3 |
25.00 |
4.64** |
5.77** |
3.63* |
2.33 |
.115 |
|
IL-4/13A |
25.77 |
1.67 |
2.71 |
1.70 |
0.80 |
-.124 |
|
IL-21 |
33.78 |
20.28** |
42.62** |
31.58** |
11.91** |
.506** |
|
IL-22 |
33.65 |
10.03* |
6.67** |
5.49* |
4.48 |
.159 |
|
IL-17A/F2a |
28.27 |
10.84** |
11.23** |
2.64 |
3.24 |
.061 |
|
IL-17C1 |
27.92 |
6.56** |
2.74* |
1.43 |
0.84 |
-.259 |
|
IL-17C2 |
30.90 |
2.08 |
1.36 |
1.06 |
0.70 |
-.349* |
|
IL-17D |
31.72 |
4.95** |
5.73** |
3.78** |
3.56* |
.259 |
|
FOXP3A |
26.19 |
2.96 |
5.15* |
4.75 |
2.52 |
.042 |
|
FOXP3B |
27.03 |
4.02** |
3.87** |
3.13** |
2.11 |
.109 |
|
TGF-β1a |
22.87 |
3.34* |
3.00** |
2.02 |
1.59 |
-.015 |
|
nIL-1F |
24.76 |
3.14* |
3.32** |
2.81* |
1.97 |
.136 |
|
IL-10A |
26.26 |
14.68** |
18.21** |
25.24** |
9.51** |
.414** |
| SOCS-3 | 22.74 | 3.44* | 8.38** | 10.24** | 5.90** | .472** |
The average base-line Cq (cCq) in control (grade 0) fish is provided for each gene studied with the exception of parasite genes, which were undetectable in control fish. Immune gene expression at each stage of clinical PKD (grade 1 to 3) was initially normalized to trout EF-1α and subsequently expressed as fold change relative to expression levels in control fish. Pearson correlation r coefficients are given relative to kidney swelling grade. Significant differences (2-tailed) between control and infection groups are shown in bold. * P < 0.05, ** P < 0.01.