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. 2013 Aug 5;8(8):e71654. doi: 10.1371/journal.pone.0071654

Figure 2. Assessment of cell-survival by MTT assay.

Figure 2

The survival of Caki-2 (VHL-wt) and 786-O (VHL-mut) RCC cell lines was assessed after treatment with increasing concentrations of the glutaminolytic inhibitor, aminooxyacetate (AOA) at 24 h (a), 48 h (b) and 72 h (c) and in glutamine-depleted medium (d). VHL-mut 786-O RCC cells were more susceptible to inhibition of glutaminolysis or glutamine depletion at all time-points (* indicates statistically significant difference from VHL-wt control by the t-test, P<0.05, n = 4).