Figure 7. IL-5 enhances IL-10 secretion from CD40L-stimulated B cells.

CD19⁺ B cells were isolated by MACS from naïve mice and cultured for five days with CD40L-expressing fibroblasts in the presence or absence of IL-5. B cells were then harvested, washed in PBS, and cultured overnight at equivalent cell concentrations (7.5×10⁵ viable cells/mL) in the presence or absence of stimulation with PMA and ionomycin. IL-10 in the culture supernatants was measured by ELISA. (A) IL-10 secretion from B cells after overnight culture with no further stimulation. (B) IL-10 secretion from B cells after overnight culture with PMA and ionomycin. Data are representative of at least three independent experiments (mean ± SEM). ***p<0.001.