Figure 11.

PHF1 and SUZ12 are co-immunoprecipitated from primary neocortical neurons, as shown by Western blot. Primary E18 cortical neurons, maintained seven days in culture, were lysed and immunoprecipitated (IP) with PHF1 (A lane 3, B lane 3), SUZ12 (A lane 5) and pre-immune (A lane 2, lane 4, B lane 2) antisera. Immune complexes were separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose. PHF1 (A) or SUZ12 (B) proteins were identified by immunoblotting with either PHF1 (A) or SUZ12 (B) antisera. The molecular weight markers are shown to the left.