Table 3.

Abundances of ¹³C in P(3HB) synthesized by R. eutropha H16 and cbbLS disruptants on fructose with addition of NaH¹³CO₃^a

R. eutropha strain	NaHCO3 addedb	P(3HB) (wt%)	13C-Abundance in P(3HB)c (%)	Increase of 13C in P(3HB) (mmol/g-P(3HB))
H16	12C	53.6 ± 2.14	1.13 ± 0.0003	-
	13C	49.5 ± 4.39	2.22 ± 0.0025	0.42 ± 0.0016
H16∆cbbLSc	12C	52.1 ± 0.91	1.10 ± 0.0001	-
	13C	48.3 ± 1.41	1.81 ± 0.0013	0.27 ± 0.0007
H16∆cbbLSp	12C	50.0 ± 2.49	1.13 ± 0.0002	-
	13C	48.3 ± 2.48	2.11 ± 0.0022	0.38 ± 0.0012
H16∆∆cbbLS	12C	27.8 ± 0.17	1.11 ± 0.0003	-
	13C	30.0 ± 0.48	1.25 ± 0.0005	0.05 ± 0.0004

^aP(3HB) biosynthesis was performed by 2-stage cultivation as described in the text.

^bAdded periodically during the second stage.

^cMeans of ¹³C/¹²C ratios calculated from isotopomer abundances of the three fragments (m/z 45, 87, and 103) derived from 3HB methyl ester.