Table 1. Oligonucleotides used.

Oligo name	Oligo sequence
Reconstruction of the Sso7d gene
Sso-W1	5′-GTATGGCGTGTGGGCAAGATGAT-3′
Sso-W2	5′-GAAAAGGACGCGCCGAAGGAGCTG-3′
Sso-C1	5′-CACACGCCATACTTTCTTGATCTTGGAGATGTCTACCTCTTTTTCTTCGCCTTTGTACTTGAACTTTACGGTTGC-3′
Sso-C2	5′-GCGCGTCCTTTTCGCTTACCGCACCGCGGCCGGTCTTGCCACCGCCCTCGTCGTAGGTGAAGGAGATCATCTTGCC-3′
Sso-C3	5′-GATTAATCCAGTGCCTTGGGACTAGTGCCACCGCCGCCCTTTTTCTGCTTCTCCAGCATCTGCAGCAGCTCCTTCG-3′
Sso-R1	5′-ACGAATTCGAGCGCAACCGTAAAGTTCAAG-3′
Sso-Xba	5′-GCGCTCTAGATTAATCTTTTTCTGCTTCTCCAGCAT-3′
Primers used for activity and processivity assays
–47M13L	5′-CGCCAGGGTTTTCCCAGTCACGACGTTGTAAAACGACGGCC-3′
–40M13LFF	5′-FAM-GTTTTCCCAGTCACGACGTTGTAAAACGACGGCC-3′
Primers used for extension efficiency assay
L30350F	5′-CCTGCTCTGCCGCTTCACGC-3′
L71-0.5R (0.5 kb)	5′-TCCGGATAAAAACGTCGATGACATTTGC-3′
L71-1R (1 kb)	5′-GATGACGCATCCTCACGATAATATCCGG-3′
L72-2R (2 kb)	5′-CCATGATTCAGTGTGCCCGTCTGG-3′
L72-5R (5 kb)	5′-CGAACGTCGCGCAGAGAAACAGG-3′
L72-8R (8 kb)	5′-GCCTCGTTGCGTTTGTTTGCACG-3′
L71-10R (10 kb)	5′-GCACAGAAGCTATTATGCGTCCCCAGG-3′
L71-12R (12 kb)	5′-TCTTCCTCGTGCATCGAGCTATTCGG-3′
L71-15R (15 kb)	5′-CTTGTTCCTTTGCCGCGAGAATGG-3′
Primers used for salt dependence PCR assay
L30350F	5′-CCTGCTCTGCCGCTTCACGC-3′
L31240R	5′-GCACAGCGGCTGGCTGAGGA-3′