Table 2. Summary of processivity analyses.
Enzyme | Microscopic processivity (PI)a | Average primer extension length (nt) [1/(1 – PI)]a |
---|---|---|
Taq(Δ289) |
0.64 ± 0.09 |
2.9 ± 0.7 |
S-Taq(Δ289) |
0.980 ± 0.002 |
51 ± 6 |
Taq |
0.95 ± 0.01 |
22 ± 3 |
S-Taq |
0.99 ± 0.02 |
104 ± 17 |
S(G)-Taq(Δ289) |
0.91 ± 0.01 |
11 ± 1 |
S(V)-Taq(Δ289) |
0.92 ± 0.01 |
12 ± 2 |
S(E)-Taq(Δ289) |
0.85 ± 0.04 |
7 ± 2 |
Pfu |
0.84 ± 0.01 |
6.4 ± 0.5 |
Pfu-S | 0.982 ± 0.001 | 55 ± 3 |
aData were analyzed as described in Materials and Methods. The standard deviation was calculated based on results from multiple sets of data.