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. 2013 Aug 5;202(3):495–508. doi: 10.1083/jcb.201302078

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© 2013 Fu and Holzbaur

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Figure 5. — Anterograde and retrograde JIP1 motor complexes are mutually exclusive. (A) Summary schematic of direct binding interactions between JIP1, Kinesin-1, and dynactin. (B) JIP1 cannot bind simultaneously to both p150^Glued and KHC stalk. Lysates from COS7 cells triple transfected with myc-JIP1, FLAG-p150^Glued, and GFP-KHC-stalk were immunoprecipitated with an anti-GFP antibody. FLAG-p150^Glued and GFP-KHC-stalk do not interact with each other either in the absence or presence of myc-JIP1. (C) JIP1 cannot bind simultaneously to both p150^Glued and KHC tail. Lysates from COS7 cells triple transfected with myc-JIP1, FLAG-p150^Glued, and GFP-KHC-tail were immunoprecipitated with an anti-GFP antibody. FLAG-p150^Glued and GFP-KHC-tail do not interact with each other either in the absence or presence of myc-JIP1. (D) JIP1 cannot bind simultaneously to p150^Glued and KHC. Lysates from COS7 cells triple transfected with myc-JIP1, FLAG-p150^Glued, and either GFP-KHC-tail or GFP-KHC-stalk were immunoprecipitated with an anti-p150^Glued antibody. Though robust levels of FLAG-p150^Glued and associated myc-JIP1 are coimmunoprecipitated, no interacting GFP-KHC-stalk or GFP-KHC-tail can be detected. (E) JIP1 can bind simultaneously to both p150^Glued and KLC. Lysates from COS7 cells triple transfected with myc-JIP1, FLAG-p150^Glued, and HA-KLC were immunoprecipitated with either an anti-FLAG or anti-HA antibody. In the absence of myc-JIP1, FLAG-p150^Glued and HA-KLC do not interact. The addition of myc-JIP1 facilitates the indirect interaction between p150^Glued and KLC as both FLAG and HA antibodies immunoprecipitate triple complexes of FLAG-p150^Glued, myc-JIP1, and HA-KLC. (F) Model of two mutually exclusive JIP1 motile complexes. The anterograde JIP1 complex activates KHC motility via direct binding to both stalk and tail domains (left) but cannot bind simultaneously to p150^Glued; KLC may remain bound via the C-terminal tail of JIP1 (Verhey et al., 2001). The retrograde JIP1 complex binds directly to p150^Glued to facilitate dynein-mediated transport and may retain autoinhibited KHC via simultaneous binding to KLC (right).