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. 2013 Aug 5;202(3):509–526. doi: 10.1083/jcb.201211148

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© 2013 Shen et al.

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Figure 1. — Visual screening of Sec15p-GFP localization in deletion strains. Strains were selected from the yeast deletion library and transformed with a GAL-SEC15-GFP construct. After 5 h of galactose induction, cells were collected and imaged by fluorescence microscopy. (A) Sec15p-GFP was concentrated at exocytic sites including bud tips and bud necks in WT cells (a), yet appeared diffuse in chc1Δ cells (b). The corresponding DIC images are shown in c and d. The appearance of Sec15p-GFP patches was quantified by scoring ∼200 cells for each strain (B). Values indicate the percentage of cells showing GFP-labeled patches at the bud tip or bud neck. Bar, 10 µm.