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. 2013 Aug 5;202(3):509–526. doi: 10.1083/jcb.201211148

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© 2013 Shen et al.

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Figure 9. — Sec9p does not interfere with the Snc2p–Sec6p interaction. GST-Snc2p (2.5 µM final concentration) was incubated with 70 nM (final concentration) His-Sec6p and increasing amounts of His-MBP-Sec9c, as indicated, and then precipitated with glutathione beads. The bead-associated protein was analyzed by SDS PAGE and Coomassie staining. Black lines indicate where lanes have been rearranged for presentation purposes.