Table 4.

Plasmids for yeast transformation or HeLa cell transfection

Name	Plasmid
pNB300	YCp50, GAL1-Sec15
PNB882	pRS306 SEC6-GFP
pNB1304	pRS306 SEC5-3xGFP
pNB1308	pRS306 SEC15-3xGFP
pNB1456	YCp50 GAL1-SEC15-GFP; a fragment of Sec15-GFP into BamH I–SalI sites of pNB300
pNB1457	pRS425 Sec4, a 392-bp Sec4 fragment was ligated into BamHI–XhoI sites
pNB1458	pRS303 Sec15-3xGFP; inserting Sec15-3xGFP-ADHterm fragment into pRS303 SalI–SacI sites
pNB1459	pRS305 Sec6-2xmCherry; inserted 1.6 kB Sec6 C-terminal fragment into XhoI–XbaI sites of pJC1-2xmCherry
pNB1460	pRS305 Sec8-2xmCherry; inserted 1 kB Sec8 C-terminal fragment into XhoI–XbaI sites of pJC1-2xmCherry
pNB1461	pRS306 Snc2-M2(75E79E82E)
pNB 1462	pCMV-myc-Exoc3
pSFNB1223	pRS416 GFP-SNC1