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. 2004 Feb 18;32(3):1232–1241. doi: 10.1093/nar/gkh275

Figure 1.

Figure 1

Splicing activity of human CELF4 deletion mutants. (A) Diagram of full-length human CELF4 protein showing RRM (dark gray) and conserved RNP2 and RNP1 motifs (black). Numbers above the diagram indicate N- and C-terminal positions of the RRMs. Horizontal lines represent the remaining portions of CELF4 deletion mutants. Residue numbers are according to accession number AAK07475. The CELF4 splice form utilized lacks the N-terminal 44 amino acids of RRM3 including the conserved RNP2 hexamer motif. The ability (+) or failure (–) to regulate splicing of an exon flanked by MSEs 1–4 is indicated. (B) Activation of chicken cTNT exon 5 inclusion by transient expression of human CELF4 full-length and truncated proteins in QT35 quail fibroblasts. % = percentage mRNAs including exon 5 as determined by RT–PCR analysis. The percentage of mRNAs including exon 5 is calculated as [CPM exon inclusion band/(CPM exon inclusion band + CPM exon exclusion band)] × 100. Black = minigene alone or not regulated by co-expressed protein; gray = regulated by co-expressed protein (correlates with + or – designation in A).