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The Mos-MAPK pathway is not sufficient for induction of the G2/M transition. Prophase extracts were supplemented with either active GST-Mos (50 μg/ml final concentration) or with PKI, and samples were frozen at the indicated times, analyzed for Plx1 and histone H1 kinase activity (A), immunoblotted for Cdc25C and pMAPK (B), and for MAPK (C).
