Figure 2.

Response of the yeast Rev1 dCMP transferase to AAF-dG and 1,N⁶-ethenoadenine DNA adducts. DNA polymerase assays were performed with 5 ng (45 fmol) of purified yeast Rev1 in the presence of all four dNTPs (N₄), or a single deoxyribonucleotide triphosphate, dATP (A), dCTP (C), dGTP (G), or dTTP (T). DNA size markers in nucleotides are indicated on the left. Quantitation of extended primers is shown below the gel. (A) Assays with DNA templates containing a site-specific AAF-dG adduct. A 17mer primer was labeled with ³²P at its 5′ end and annealed to the damaged template with the primer 3′ end terminating right before the lesion as shown on the top. (B) Assays with DNA templates containing a site- specific 1,N⁶-ethenoadenine adduct. A 20mer primer was labeled with ³²P at its 5′ end and annealed to the damaged template with the primer 3′ end terminating right before the lesion as shown on the top.