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. 2013 Apr 23;14:134. doi: 10.1186/1471-2105-14-134

Figure 2.

Figure 2

DIC shear direction estimation procedure. DIC shear direction estimation from image of spherical bead of diameter 1 [μm]. An estimated orientation aμ = 43.52 [deg] (red line) was calculated with a rotated linear structuring element defined by l = 20 [pixels] and n = 180. Scale bar corresponds to 1 [μm].