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. 2004 Feb 18;32(3):1242–1250. doi: 10.1093/nar/gkh281

Figure 1.

Figure 1

(A) Mer1p domains and fusion constructs used in this study. Mer1p contains a C-terminal KH domain RNA-binding motif and an N-terminal domain required for activating splicing. CTMER has the MS2 Coat gene fused to the N-terminus of the entire MER1 gene, whereas CTAD has the MS2 Coat gene fused to the activation domain fragment of MER1. The MS2 Coat construct serves as a control that produces unfused MS2 Coat protein. The AD construct codes for the N-terminal activation domain from MER1, and the KH construct codes for the C-terminal fragment of Mer1p which contains the KH domain and short C-terminal peptide. (B) The sequences of the MS2 operator and MER1 splicing enhancer elements used to modify the actin intron.