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. 2004 Feb 18;32(3):1242–1250. doi: 10.1093/nar/gkh281

Figure 2.

Figure 2

The KH domain of Mer1p can be replaced with another RNA-binding domain. Primer extension analysis was used to measure splicing of modified actin RNAs produced in vivo. Actin RNAs were expressed from a plasmid and contained a G5A 5′ splice site mutation to reduce splicing efficiency and either the Mer1p enhancer element or the MS2 operator element near the 5′ splice site. Yeast cells also included one of the plasmids from Figure 1. Lanes 2 and 5 are replicates. The dark band between the 262 and 345 nt markers in lanes 1–5 results from a primer extension stop at the MS2 operator hairpin. Phosphorimaging quantitation of splicing efficiency (percent spliced) is calculated by the formula S/(S + U) × 100 where S = spliced and U = unspliced. The average values for duplicate samples are reported.