Figure 3.

Over-expression of the activation domain of Mer1p has a dominant-negative effect on full-length Mer1p. Yeast were transformed with a MER1 expression plasmid (pMER1) or a control plasmid lacking MER1 (pControl). Additionally, the yeast contained the KH domain plasmid (KH), the activation domain plasmid (AD) or a control plasmid (C) that lacks any of the MER1 domains. Total RNA was isolated and splicing of the AMA1 pre-mRNA was measured by primer extension on duplicate samples. Splicing efficiencies for each sample are reported below each lane.