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. 2004 Feb 18;32(3):1159–1165. doi: 10.1093/nar/gkh285

Figure 2.

Figure 2

An additional PatpB-329 NEP promoter is accurately recognized in vitro. (A) Primer extension analysis to map the 5′ end of atpB in vitro transcripts from ΔrpoA plastid extracts. For reference, the same end-labeled primer was used to generate a DNA sequence ladder. (B) DNA sequences surrounding the atpB NEP transcription initiation sites. Bold nucleotides denote transcription initiation sites (+1). Putative YRTA core motifs are shaded with gray boxes and the –35 PEP promoter motif is underlined.