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. 2004 Feb 18;32(3):1159–1165. doi: 10.1093/nar/gkh285

Figure 4.

Figure 4

Expression analysis of NsRpoTp in mutant NtKL plants. (A) Western blot analysis of 10 µg of plastidial proteins separated in 6.5% SDS–polyacrylamide gels. Monoclonal Anti-FLAG antibody detected 120 (asterisk) and 110 kDa (arrowhead) proteins. The 120 kDa protein is probably non-specific because it was also detected in wild-type extracts. (B) Northern blot analysis of 50 µg of total RNA separated in 1% agarose–formaldehyde gels. Ethidium bromide stained gel image (EtBr) is shown as a loading control (bottom). The RNA blot was hybridized with a single-stranded full-length NsRpoTp antisense DNA probe. Note the weak 3.7 kb signal detected in the wild-type (lane 4) which most likely represents endogenous NtRpoTp transcripts.