Table 1. Comparison of transcript abundance in RpoTp overexpressing NtKL and wild-type plants.
| |
atpB |
accD |
clpP |
|||||||
|---|---|---|---|---|---|---|---|---|---|---|
| |
P-255 |
P-289 |
P-329 |
P-502 |
P-611 |
P-129 |
P-53 |
P-95 |
P-173 |
P-511 |
| PEP | NEP-I | NEP-I | PEP | PEP | NEP-I | NEP-II | PEP | NEP-I | NEP-I | |
| AtRpoTp | ||||||||||
| NtKL85-7 |
69 ± 2 |
33 ± 1 |
212 ± 4 |
93 ± 5 |
59 ± 8 |
– |
99 ± 0.5 |
79 ± 18 |
11 ± 4 |
0 |
| NtKL85-8 |
73 ± 3 |
26 ± 5 |
143 ± 30 |
87 ± 3 |
56 ± 7 |
– |
100 ± 3 |
37 ± 3 |
3 ± 1 |
0 |
| NtKL85-9 |
52 ± 6 |
43 ± 9 |
209 ± 2 |
54 ± 17 |
48 ± 10 |
– |
102 ± 4 |
43 ± 5 |
2 ± 1.5 |
0 |
| NtKL85-12 |
108 ± 4 |
12 ± 8 |
0.2 ± 0.1 |
104 ± 8 |
109 ± 8 |
– |
98 ± 2 |
101 ± 3 |
99 ± 2 |
0 |
| ΔrpoB |
0 |
100 |
100 |
0 |
0 |
– |
157 ± 4 |
0 |
2 ± 1 |
100 |
| wt |
100 |
19 ± 4 |
0.5 ± 0.4 |
100 |
100 |
– |
100 |
100 |
100 |
0 |
| NsRpoTp | ||||||||||
| NtKL197-6 |
34 ± 5 |
100 ± 8 |
253 ± 2 |
41 ± 3 |
19 ± 11 |
8 ± 2 |
98 ± 0.5 |
91 ± 3 |
68 ± 1 |
22 ± 4 |
| NtKL197-4 |
31 ± 3 |
103 ± 6 |
248 ± 4 |
36 ± 4 |
38 ± 9 |
5 ± 3 |
97 ± 1 |
92 ± 2 |
63 ± 3 |
27 ± 2 |
| NtKL199-7 |
68 ± 9 |
73 ± 10 |
118 ± 6 |
62 ± 11 |
58 ± 5 |
1 ± 0.4 |
110 ± 5 |
100 ± 1 |
73 ± 4 |
2 ± 2 |
| ΔrpoA |
0 |
100 |
100 |
0 |
0 |
100 |
98 ± 2 |
0 |
1 ± 0.5 |
100 |
| wt | 100 | 50 ± 5 | 0 | 100 | 100 | 0 | 100 | 100 | 100 | 0 |
Summary of results obtained by three independent primer extension analyses. Percentages (%) with standard error values in respect to wild-type (PEP) and ΔrpoB (NEP) plant transcript levels are given. Bold type represents values set to 100% for each promoter. NEP-I, type I NEP promoter; NEP-II, type II NEP promoter.