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(A) Schematic representation of dsCUG. (B) Denaturing polyacrylamide gel electrophoresis of (1) the total 15N-labelled RNA synthesized in a 15 ml in vitro transcription reaction and (2) (CUG)97 RNA after purification and refolding. The arrow points to the full-length transcript. (C) A 1H–15N HSQC spectrum of a 200 µM (CUG)97 sample taken at 15°C; G and U denote the cross-peaks of the imino groups of the G and U nucleotides of (CUG)97.
