Figure 8.

Overrepresented motifs upstream of genes in clusters peaking primarily at 72 hrs post-infection. A. Clusters peaking primarily at 72 hrs post-infection. B. Overrepresented motifs upstream of genes in these clusters. Nine representative upstream regions are shown out of 105 searched. Upstream regions for each of these genes were mined for overrepresented motifs (see Materials and Methods). The upstream regions of genes in clusters peaking primarily at 72 hours share four overrepresented motifs. Two of these motifs are similar to previously identified ApiAP2 binding sites. One binding site is E2F-like. The remaining site is similar to the G-box noted in other apicomplexans, which we have demonstrated is an ApiAP2 binding site in C. parvum.