Figure 2. The effects of overexpression or absence of C/ebpβ on Arf induction by Tgfβ.

(A). β-galactosidase activity in Arf ^lacZ/lacZ MEFs showing the effects of ectopically-expressed C/ebpβ (LAP form) on Arf induction following 48 hour exposure to Tgfβ. Significant increase (*) and decrease (#) of Arf^lacZ expression is represented in the figure. *, #, p<0.05. (B) Representative western blot for the indicated proteins using lysates from wild type MEFs, exposed to 48 hours of Tgfβ (T) and vehicle (V) after transduction using Gfp- or C/ebpβ (LAP form)-expressing retrovirus. (C) qRT-PCR using total RNA isolated from C/ebpβ ^+/+ and C/ebpβ ^−/− MEFs exposed to vehicle (V) or Tgfβ (T) for 48 hours. Differences in transcript level between Tgfβ- and vehicle-treated C/ebpβ ^+/+ MEFs are significant [p<0.05 (*)]. Differences in transcript level between vehicle-treated C/ebpβ ^+/+ and C/ebpβ ^−/− MEFs are significant, too [p<0.05 (**)]. (D) Representative western blot for the indicated proteins using lysates from C/ebpβ ^+/+ and C/ebpβ ^−/− MEFs exposed to vehicle (V) or Tgfβ (T) for 48 hours.