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. 2013 Aug 5;8(8):e70371. doi: 10.1371/journal.pone.0070371

Figure 3. Loss of C/ebpβ increases Arf mRNA expression in vitreous of developing eye.

Figure 3

(A). qRT-PCR analysis using total RNA isolated from the vitreous (V), lens (L) and retina (R) from E13.5 WT mouse embryos. Expression was normalized to that of Gapdh. (B) qRT-PCR analysis using total RNA isolated from the vitreous from E13.5 C/ebpβ +/+ and C/ebpβ −/− mouse embryos. Expression was normalized to that of Gapdh. (C) Arf expression is limited to previously identified sites in C/ebpβ −/− mice during development. (a, b) Representative photomicrographs of hematoxylin- and eosin-stained and X-Gal stained slides of P1 mouse eye of the indicated genotype. Note that Arf-expressing cells are limited to the vitreous (blue staining) in the Arf lacZ/lacZ, C/ebpβ −/− embryo, similar to the littermate Arf lacZ/lacZ, C/ebpβ +/+ control embryo. (c,d) Representative whole-mount, E13.5 embryo from mice of the indicated genotype, following X-gal staining. Note that Arf-expressing cells are limited to the umbilical artery (arrow) in the Arf lacZ/lacZ, C/ebpβ −/− embryo, similar to its littermate Arf lacZ/lacZ, C/ebpβ +/+ control embryo. K, kidney; B, bladder. (D). Representative photomicrographs of hematoxylin- and eosin-stained slides of E15.5 embryos showing there is no primary vitreous hyperplasia in C/ebpβ −/− embryos. Arrows denote the cellular area of the primary vitreous.