Figure 2. ERF6 gene expression relative to actin genes analyzed by qRT-PCR.

A) In wild-type (WT) Arabidopsis seedlings (grown on MS plates) pretreated with the calcium channel blocker La³⁺ followed by H₂O₂ treatment. LaCl₃ was added at a final concentration of 2 mM, H₂O₂ was added at a final concentration of 50 mM. B, C and D). After spraying 4-week-old soil-grown Arabidopsis wild-type or mutant (myc2, zat10 and rbohD, respectively) plants with H₂O₂ (6h). Three independent biological replicates (with 20 plants each) were used for each treatment. Error bars represent standard deviations. Asterisks indicate significant (P<0.05) differences in treated plants compared to mock-treated plants (A) or between mutant and WT (B-D).