Table 2. Differential gene expression determined by qRT-PCR in erf6 mutant compared to wild-type Arabidopsis plants under H₂O₂ treatment.

Functionalcategory	AGI Number	Gene	erf6 to wild-type ratio*	P value**	Predicted/known location of gene product
ROS Generation
	At5g47910	RbohD	4.56±1.82	0.039	Membrane
	At1g09090	RbohB	2.41±1.36	0.067	Membrane
Signaling
	At4g33630	EX1	−6.78±1.63	0.059	Chloroplast
	At3g25250	OXI1	3.25±0.19	0.003	Unknown
	At2g43790	MAPK6	4.17±0.01	0.003	Various
	At3g45640	MAPK3	3.13±1.73	0.008	Various
	At5g13080	WRKY75	2.29±0.96	0.098	Nucleus
	At1g80840	WRKY40	2.68±0.66	0.017	Nucleus
	At5g59820	ZAT12	7.10±2.01	0.025	Nucleus
	At4g18880	HSFA4A	2.89±0.28	0.004	Nucleus
	At4g17750	HSF1	5.23±2.49	0.042	Nucleus
Antioxidant and defense
	At3g52880	MDAR1	3.37±1.18	0.063	Peroxisome
	At5g03630	MDAR2	3.44±0.83	0.022	Cytosol
	At3g09940	MDAR3	−9.60±2.89	0.005	Cytosol
	At1g19570	DHAR1	6.41±1.46	0.019	Chloroplast
	At4g09010	APX4	2.47±0.78	0.013	Microsome
	At1g20630	CATALASE1	4.20±0.11	0.013	Various
	At4g35090	CATALASE2	3.39±1.04	0.057	Peroxisome
	At1g20620	CATALASE3	−12.83±2.77	0.028	Various
	At4g26850	VTC2	−8.77±3.12	0.011	Unknown
	At3g46230	HSP17	1.99±0.30	0.029	Unknown
	At5g44420	PDF1.2	5.02±0.67	0.004	Cell wall

The values represent the average of three biological replicates.

^*Fold difference (2-fold or more) of gene expression in erf6 plants compared to wild-type plants at 6 hours after H₂O₂ treatment ± SD. Negative signs indicate reduced expression in erf6 compared to wild-type plants.

^**P value, Student’s t test was used to calculate probabilities and to determine significant differences.