Table 2. Differential gene expression determined by qRT-PCR in erf6 mutant compared to wild-type Arabidopsis plants under H2O2 treatment.
Functionalcategory | AGI Number | Gene | erf6 to wild-type ratio* | P value** | Predicted/known location of gene product |
ROS Generation | |||||
At5g47910 | RbohD | 4.56±1.82 | 0.039 | Membrane | |
At1g09090 | RbohB | 2.41±1.36 | 0.067 | Membrane | |
Signaling | |||||
At4g33630 | EX1 | −6.78±1.63 | 0.059 | Chloroplast | |
At3g25250 | OXI1 | 3.25±0.19 | 0.003 | Unknown | |
At2g43790 | MAPK6 | 4.17±0.01 | 0.003 | Various | |
At3g45640 | MAPK3 | 3.13±1.73 | 0.008 | Various | |
At5g13080 | WRKY75 | 2.29±0.96 | 0.098 | Nucleus | |
At1g80840 | WRKY40 | 2.68±0.66 | 0.017 | Nucleus | |
At5g59820 | ZAT12 | 7.10±2.01 | 0.025 | Nucleus | |
At4g18880 | HSFA4A | 2.89±0.28 | 0.004 | Nucleus | |
At4g17750 | HSF1 | 5.23±2.49 | 0.042 | Nucleus | |
Antioxidant and defense | |||||
At3g52880 | MDAR1 | 3.37±1.18 | 0.063 | Peroxisome | |
At5g03630 | MDAR2 | 3.44±0.83 | 0.022 | Cytosol | |
At3g09940 | MDAR3 | −9.60±2.89 | 0.005 | Cytosol | |
At1g19570 | DHAR1 | 6.41±1.46 | 0.019 | Chloroplast | |
At4g09010 | APX4 | 2.47±0.78 | 0.013 | Microsome | |
At1g20630 | CATALASE1 | 4.20±0.11 | 0.013 | Various | |
At4g35090 | CATALASE2 | 3.39±1.04 | 0.057 | Peroxisome | |
At1g20620 | CATALASE3 | −12.83±2.77 | 0.028 | Various | |
At4g26850 | VTC2 | −8.77±3.12 | 0.011 | Unknown | |
At3g46230 | HSP17 | 1.99±0.30 | 0.029 | Unknown | |
At5g44420 | PDF1.2 | 5.02±0.67 | 0.004 | Cell wall |
The values represent the average of three biological replicates.
Fold difference (2-fold or more) of gene expression in erf6 plants compared to wild-type plants at 6 hours after H2O2 treatment ± SD. Negative signs indicate reduced expression in erf6 compared to wild-type plants.
P value, Student’s t test was used to calculate probabilities and to determine significant differences.