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. 2013 Aug 5;8(8):e70952. doi: 10.1371/journal.pone.0070952

Figure 1. Generation of experimental mice and analysis of body weight.

Figure 1

A. Diagram showing the breeding design. First, lpr/+;Tet/DN-GSK-3 and lpr/+ mice (F1) were obtained by crossing lpr/lpr mice with Tet/DN-GSK-3 or wt mice respectively (F0). Then, lpr/+;Tet/DN-GSK-3 and lpr/+ mice were combined to obtain 12 possible genotypes among which we obtained the 6 experimental groups: wt, Tet/DN-GSK-3, lpr/+, lpr/+;Tet/DN-GSK-3, lpr/lpr and lpr/lpr;Tet/DN-GSK-3. B. Identification of the different genotypes by PCR to detect the CamKII-tTA and the DN-GSK-3 transgenes and the presence of the lpr mutation. Line 1: wt; line 2: Tet/DN-GSK-3; line 3: lpr/+; line 4: lpr/+; Tet/DN-GSK-3; line 5: lpr/lpr; line 6: lpr/lpr; Tet/DN-GSK-3. Analysis of the transgene expression by western blot in striatum the six experimental genotypes and example of immunohistochemistry of the reporter β-gal in striatum (St) of Tet/DN-GSK-3 mice. Scale bar corresponds to 100 µm. ac, anterior commissure; GP, globus pallidus. C. Body weight of all resulting genotypes was measured at the age of 2.5 months. One-way ANOVA test followed by Bonferroni post-hoc test was applied to determine the level of significance. The number of analyzed animals is indicated in the graph. Only lpr/lpr mice were significantly different than wt mice (* p<0.05).