Figure 2.
Effect of cell synchronization on targeting efficiency. (A) Syn chronization of cells in S-phase with 2 mM thymidine for 24 h. (B) Number of random and targeted insertions obtained with each targeting construct per 107 cells in S-phase synchronized cells. No colony was obtained either with a control vector containing only nls or with no vector thereby indicating that 8-AG resistance does not occur in response to the nls or cell synchronization process. (C) Percent targeted colonies in thymidine-synchronized cells with different targeting constructs. Columns with different superscripts are different at P < 0.001. Means ± SE.