Figure 6. Dynamin-2 and actin polymerization regulate the fusion pore expansion and quantal size in BCC.

Chromaffin cells were incubated with 4 µM CytoD during 10 minutes at 37°C. After that the exocytosis was evoked with 10 µM DMPP. A–C: Data show average values ± SEM of Q (A), t_1/2 (B) and foot duration (C) of amperometric spikes induced by 10 µM DMPP in cells transfected with pEGFP (n = 27), Dyn2K44A (n = 13) or iRNADyn2 (n = 16). All amperometric parameter values correspond to the median values of the events from individual cells, which were subsequently averaged per treatment group. Thus, n correspond to the number of cells in each treatment group. Note that the CytoD treatment (grey bars) significantly increased Q, t_1/2 and foot duration of the exocytotic events in cells transfected with pEGFP, without additional effects in cells transfected with Dyn2K44A or iRNADyn2. * p<0.05 compared with the untreated cells (Kruskal-Wallis test).