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. 2013 Aug 5;8(8):e70638. doi: 10.1371/journal.pone.0070638

Table 1. Amperometric parameters of exocytotic events induced by 10 µM DMPP in cells transfected with pEGFP, UnR-iRNA, iRNADyn2, Dyn2WT or Dyn2K44A.

pEGFP UnR-iRNA iRNADyn2 Dyn2WT Dyn2K44A
Number of events 17.7±1.7 24.2±3.6 23.0±3.2 20.2±2.4 21.5±3.1
Q (pC) 0.83±0.08 0.87±0.09 1.33±0.08* 0.78±0.09 1.33±0.12
t1/2 (ms) 13.6±1.1 13.0±0.8 19.4±1.6* 11.5±1.0 18.4±1.8
Imax (pA) 51.5±3.8 55.1±5.1 59.4±5.6 65.3±7.9 62.4±3.7
Foot frequency(%) 63.3±5.5 57.9±3.7 57.8±3.6 61.6±10 63.4±8.3
Foot amplitude(pA) 12.4±1.3 13.6±1.0 11.3±0.9 11.6±1.1 12.1±1.6
Foot duration(ms) 14.3±1.4 11.0±0.6 22.4±2.1* 11.8±1.1 19.4±1.8
Number ofcells 35 28 29 15 18

Data are means ± SEM of averages, where n is the number of cells. Imax corresponds to the spike amplitude.

*

p<0.05 compared with pEGFP-transfected cells;

p<0.05 compared with Dyn2 WT-transfected cells (Kruskal-Wallis test).