Table 1. Amperometric parameters of exocytotic events induced by 10 µM DMPP in cells transfected with pEGFP, UnR-iRNA, iRNADyn2, Dyn2WT or Dyn2K44A.
pEGFP | UnR-iRNA | iRNADyn2 | Dyn2WT | Dyn2K44A | |
Number of events | 17.7±1.7 | 24.2±3.6 | 23.0±3.2 | 20.2±2.4 | 21.5±3.1 |
Q (pC) | 0.83±0.08 | 0.87±0.09 | 1.33±0.08* | 0.78±0.09 | 1.33±0.12† |
t1/2 (ms) | 13.6±1.1 | 13.0±0.8 | 19.4±1.6* | 11.5±1.0 | 18.4±1.8† |
Imax (pA) | 51.5±3.8 | 55.1±5.1 | 59.4±5.6 | 65.3±7.9 | 62.4±3.7 |
Foot frequency(%) | 63.3±5.5 | 57.9±3.7 | 57.8±3.6 | 61.6±10 | 63.4±8.3 |
Foot amplitude(pA) | 12.4±1.3 | 13.6±1.0 | 11.3±0.9 | 11.6±1.1 | 12.1±1.6 |
Foot duration(ms) | 14.3±1.4 | 11.0±0.6 | 22.4±2.1* | 11.8±1.1 | 19.4±1.8† |
Number ofcells | 35 | 28 | 29 | 15 | 18 |
Data are means ± SEM of averages, where n is the number of cells. Imax corresponds to the spike amplitude.
p<0.05 compared with pEGFP-transfected cells;
p<0.05 compared with Dyn2 WT-transfected cells (Kruskal-Wallis test).