Figure 2. Cellular uptake of pirfenidone NPs: (A) Primary corneal fibroblasts were incubated with Nile red loaded NPs, washed at different time points and the presence of red fluorescence of Nile red within the cells was detected by fluorescence microscopy.
Nucleus was stained with DAPI. (B) Corrected total cell fluorescence (CTCF) measured at different time points. Data expressed as Mean ± SD; *p<0.05 vs 5 Min, # p<0.05 vs 15 Min.