Figure 4. Effects of treatment with IFN-γ on MG-63, U-2OS and Saos-2 osteosarcoma cells with western blot analysis.

GAPDH was used as an internal control. Histogram represents densitometric analysis of the ratio of B7-H3 and GAPDH bands. Experiments were repeated at least 3 times and the mean value was calculated. *p<0.05 denotes significant differences between osteosarcoma cells and those treated with IFN-γ. p values were determined by Paired Student’s t test.