Figure 5. Overexpression or silencing of B7-H3 expression regulates the expression of MMP-2 in osteosarcoma cells with western blot analysis.

GAPDH was used as an internal control. Histogram represents densitometric analysis of the ratio of B7-H3, MMP-2 and GAPDH bands. Experiments were repeated at least 3 times and the mean value was calculated. *p<0.05, compared with control cells. p values were determined by Paired Student’s t test.