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. 2013 Aug 5;8(8):e70603. doi: 10.1371/journal.pone.0070603

Figure 3. Determination of the number of genes required to calculate a hypothetical normalization factor.

Figure 3

Pairwise variation (Vn/n+1) analysis was carried out to determine the number of reference genes required for accurate normalization. An asterisk indicates the lowest V value in each experiment. An arrowhead indicates the minimum number of genes required to pass the suggested cut-off value (0.15) [4]. See Table 2 for experiment description.