Table 1. Information of selected genes after evaluation, and characteristics of PCR products and primers used in this analysis.
| Fragaria x ananassagene ID | Fragaria vesca orthologe (a) | Gene description | Oligo orientation | Sequence (5′-3′) | Primer melting temp(°C) (b) | Product size (bp) | Optimal anealing (°C) (c) | PCR product melting temp (°C) (d) | PCR efficiency ± SD (e) | Ref (f) |
| FaGAPDH2 | gene07104 | Glyceraldehyde-3-phosphate dehydrogenase | sense chain | CCCAAGTAAGGATGCCCCCATGTTCG | 82,1 | 117 | 65 | 85 | 1,769±0,028 | [26] |
| anti-sense chain | TTGGCAAGGGGAGCAAGACAGTTGGTAG | 81,2 | ||||||||
| FaUBQ1 | gene08438 | Ubiquitin E2 | sense chain | CCCATCTCCGACAACCGCCACATCTAAA | 83,1 | 130 | 66 | 89,5 | 1,727±0,028 | |
| anti-sense chain | CCGCCGCCCCAATCTTCTGTACTCC | 82,7 | ||||||||
| FaGAPDH1 | gene18492 | Glyceraldehyde-3-phosphate dehydrogenase | sense chain | GGCTTCTATCTCAACCGGCTCGTCTT | 77,7 | 121 | 65 | 85 | 1,925±0,025 | [51] |
| anti-sense chain | CTTCCCACTGCTCCCTGATCTCTGATAC | 77,3 | ||||||||
| FaEF1α | gene28639, gene28622, gene23217 | Elongation factor 1-alpha | sense chain | TGGATTTGAGGGTGACAACATGA | 73,1 | 145 | 65 | 87 | 1,798±0,028 | [27] |
| anti-sense chain | GTATACATCCTGAAGTGGTAGACGGAGG | 73,3 | ||||||||
| FaTUBα | gene01798, gene05604, gene03851, gene26908 | Tubulin alpha | sense chain | CATGGCTTGCTGTTTGATGTACCGTG | 78,5 | 156 | 65 | 87 | 1,765±0,027 | |
| anti-sense chain | GGGACAACAGTGGGTGGCTGGTAGTT | 79,1 | ||||||||
| FaTUBβ | gene07781, gene13266, gene20192, gene08531, gene18775 | Tubulin beta | sense chain | ACACTGTTGTGGAGCCTTACAATGCTAC | 74,7 | 172 | 65 | 85,5 | 1,773±0,024 | |
| anti-sense chain | GACATTGTTGCGGAGATCAAGTGATT | 74,8 | ||||||||
| FaACTIN | gene26612, gene18390, gene22626, gene18570, gene14112, gene01836 | Actin | sense chain | GGGCCAGAAAGATGCTTATGTCGG | 77 | 152 | 65 | 85 | 1,801±0,029 | [28] |
| anti-sense chain | GGGCAACACGAAGCTCATTGTAGAAG | 76,2 | ||||||||
| FaCHC1 | gene25887 | SWI/SNF complex component | sense chain | CATCTGTTTCCGCCACAACCTATACAT | 75,1 | 156 | 65 | 83,5 | 1,762±0,03 | |
| anti-sense chain | TTTGTTTTTCTCTGAGTTGGCCATTAGA | 74,1 | ||||||||
| FaMT1 | gene10517 | S-adenosyl-L-methionine-dependent methyltransferase | sense chain | AGGAGATAAGATAGCATTCGAAGTACCC | 71,5 | 153 | 65 | 83,5 | 1,782±0,028 | |
| anti-sense chain | CTGTACTTAGGATCACAAGGCTTGAAC | 70,9 | ||||||||
| FaBZIP1 | gene17796 | Basic leucine zipper transcription factor | sense chain | AGGGTCAACAAAACCAGAATGGGGATAA | 77,7 | 151 | 65 | 85,5 | 1,712±0,027 | |
| anti-sense chain | CTGCGTTCCAGCTCTGAAATGTATTGC | 77,8 | ||||||||
| FaTIM1 | gene17570 | Mitochondrial import inner membrane translocase | sense chain | GCTCCGCCACTTACGCCGCTAATTT | 80,2 | 100 | 65 | 86,5 | 1,811±0,028 | |
| anti-sense chain | AGATCATCAGGCCCCGTCTTTCTCGTTA | 80 | ||||||||
| FaFHA1 | gene17571 | SMAD/FHA domain-containing protein | sense chain | ATTGCATGCTAAGTTGGTGGAACAGTAT | 73,9 | 179 | 65 | 85,5 | 1,744±0,025 | |
| anti-sense chain | GACCCTTAGACCTTGTGTTGATGACAAA | 74,6 | ||||||||
| FaRIB413 | gene33863 | RNA interspacer (16S–23S) region | sense chain | ACCGTTGATTCGCACAATTGGTCATCG | 83,4 | 149 | 65 | 91 | 1,784±0,032 | [29] |
| anti-sense chain | TACTGCGGGTCGGCAATCGGACG | 81,9 | ||||||||
| FaWRKY1 | gene07210 | WRKY75 like transcription factor | sense chain | ACAGCAGTAAGATTAGGGATGAAGAAGGGAG | 76,2 | 196 | 65 | 85,5 | 1,824±0,025 | [30] |
| anti-sense chain | GCTTCTTCACATTGCAACCCTGATGCGTG | 83,8 |
(a) Accession number of genes found in Fragaria vesca genome (http://www.strawberrygenome.org/and http://www.rosaceae.org/) that may be amplified by the designed primer pairs. (b) Theoretical melting temperatures calculated by Oligo Primer Analisys software version 6.65 for each primer. (c) Recommended optimal annealing temperature was calculated by gradient PCR and subsequent PCR efficiency optimization. (d) PCR-product melting temperature as determined by melting curves (e) PCR efficiencies were calculated by LigRegPCR. (f) Known references for genes previously analyzed or described.