Table 3. Summary of statistics evaluating stability of gene expression.
| Mean b | SD | CV (%) | Slope c | Intercept | Stability index d | Mean b | SD | CV (%) | Slope c | Intercept | Stability index d | ||||
| Ripening-Camarosa-Fruit (n = 20) a | Fungal infection-Andana-Petiole (n = 28) | ||||||||||||||
| * | FaRIB413 | 8,341 | 0,239 | 2,860 | 0,004 | 8,329 | 0,011 | * | FaGAPDH1 | 26,129 | 0,632 | 2,418 | 0,004 | 26,143 | 0,009 |
| * | FaCHC1 | 23,085 | 0,201 | 0,869 | 0,021 | 23,024 | 0,018 | * | FaGAPDH2 | 18,484 | 0,374 | 2,024 | 0,025 | 18,585 | 0,051 |
| * | FaTUBβ | 22,334 | 0,359 | 1,609 | 0,015 | 22,289 | 0,024 | * | FaACTIN | 23,640 | 0,428 | 1,812 | 0,030 | 23,760 | 0,054 |
| FaACTIN | 23,894 | 0,309 | 1,294 | 0,144 | 24,326 | 0,186 | * | FaEF1α | 18,780 | 0,417 | 2,219 | 0,026 | 18,886 | 0,059 | |
| FaTIM1 | 22,602 | 0,359 | 1,587 | 0,151 | 23,054 | 0,239 | * | FaMT1 | 23,992 | 0,506 | 2,108 | 0,028 | 23,879 | 0,060 | |
| FaMT1 | 25,622 | 0,449 | 1,753 | 0,143 | 25,193 | 0,251 | * | FaFHA1 | 24,251 | 0,570 | 2,349 | 0,036 | 24,107 | 0,085 | |
| FaEF1α | 17,406 | 0,413 | 2,371 | 0,161 | 17,889 | 0,382 | FaTUBβ | 21,575 | 0,374 | 1,734 | 0,090 | 21,216 | 0,155 | ||
| FaFHA1 | 23,258 | 0,643 | 2,765 | 0,204 | 23,870 | 0,564 | FaCHC1 | 26,339 | 0,404 | 1,536 | 0,120 | 26,816 | 0,184 | ||
| FaTUBα | 22,899 | 1,174 | 5,128 | 0,556 | 24,567 | 2,851 | FaBZIP1 | 26,410 | 0,446 | 1,688 | 0,110 | 25,971 | 0,185 | ||
| FaBZIP1 | 30,089 | 1,485 | 4,936 | 0,607 | 28,270 | 2,994 | FaTIM1 | 26,864 | 0,481 | 1,790 | 0,116 | 26,401 | 0,207 | ||
| FaUBQ1 | 26,677 | 1,249 | 4,680 | 0,812 | 29,113 | 3,800 | FaUBQ1 | 27,650 | 0,642 | 2,322 | 0,115 | 27,085 | 0,266 | ||
| FaGAPDH2 | 17,073 | 1,071 | 6,274 | 0,622 | 18,939 | 3,903 | FaRIB413 | 8,790 | 0,444 | 5,053 | 0,061 | 9,036 | 0,310 | ||
| FaGAPDH1 | 24,080 | 1,715 | 7,120 | 1,115 | 27,425 | 7,939 | FaTUBα | 20,281 | 0,595 | 2,931 | 0,211 | 19,436 | 0,620 | ||
| Fungal infection-Camarosa-Fruit (n = 20) | Hormonal treatment-Camarosa-Young in-vitro plant (n = 36) | ||||||||||||||
| * | FaGAPDH1 | 23,530 | 0,316 | 1,345 | 0,005 | 23,545 | 0,007 | * | FaGAPDH1 | 25,817 | 0,479 | 1,856 | 0,024 | 25,938 | 0,045 |
| * | FaTUBα | 21,462 | 0,322 | 1,499 | 0,019 | 21,518 | 0,028 | * | FaUBQ1 | 28,954 | 0,518 | 1,789 | 0,026 | 29,085 | 0,047 |
| * | FaUBQ1 | 25,599 | 0,405 | 1,583 | 0,047 | 25,458 | 0,074 | * | FaGAPDH2 | 19,183 | 0,278 | 1,451 | 0,038 | 18,993 | 0,055 |
| FaGAPDH2 | 16,274 | 0,331 | 2,031 | 0,062 | 16,090 | 0,125 | * | FaRIB413 | 8,838 | 0,523 | 5,912 | 0,016 | 8,760 | 0,093 | |
| FaACTIN | 23,539 | 0,314 | 1,335 | 0,136 | 23,133 | 0,181 | FaCHC1 | 26,297 | 0,482 | 1,832 | 0,080 | 25,895 | 0,147 | ||
| FaEF1α | 16,556 | 0,250 | 1,510 | 0,130 | 16,166 | 0,196 | FaTUBα | 23,058 | 0,586 | 2,542 | 0,093 | 22,591 | 0,237 | ||
| FaTIM1 | 24,031 | 0,372 | 1,549 | 0,131 | 23,638 | 0,203 | FaFHA1 | 25,649 | 0,615 | 2,396 | 0,101 | 25,145 | 0,242 | ||
| FaCHC1 | 23,929 | 0,467 | 1,953 | 0,121 | 23,568 | 0,235 | FaEF1α | 18,593 | 0,442 | 2,375 | 0,119 | 17,996 | 0,284 | ||
| FaTUBβ | 21,668 | 0,387 | 1,784 | 0,133 | 21,271 | 0,236 | FaMT1 | 25,669 | 0,726 | 2,829 | 0,165 | 24,846 | 0,466 | ||
| FaBZIP1 | 27,780 | 0,478 | 1,719 | 0,164 | 27,288 | 0,282 | FaTIM1 | 27,336 | 0,800 | 2,928 | 0,176 | 26,457 | 0,514 | ||
| FaFHA1 | 23,606 | 0,545 | 2,308 | 0,213 | 22,969 | 0,490 | FaTUBβ | 23,573 | 0,775 | 3,286 | 0,218 | 22,484 | 0,716 | ||
| FaRIB413 | 8,635 | 0,323 | 3,736 | 0,158 | 8,161 | 0,590 | FaBZIP1 | 27,459 | 0,845 | 3,079 | 0,262 | 26,150 | 0,806 | ||
| FaMT1 | 25,910 | 0,745 | 2,876 | 0,425 | 24,635 | 1,222 | FaACTIN | 25,122 | 0,979 | 3,899 | 0,325 | 23,499 | 1,265 | ||
| Fungal infection-Camarosa-Crown (n = 32) | Hormonal treatment-Chandler-Cellular suspensions (n = 24) | ||||||||||||||
| * | FaUBQ1 | 27,734 | 0,486 | 1,752 | 0,037 | 27,567 | 0,065 | * | FaTIM1 | 25,850 | 0,974 | 3,766 | 0,003 | 25,862 | 0,013 |
| * | FaRIB413 | 7,873 | 0,241 | 3,057 | 0,027 | 7,752 | 0,083 | * | FaGAPDH2 | 17,889 | 0,300 | 1,679 | 0,013 | 17,843 | 0,022 |
| FaGAPDH1 | 25,569 | 0,453 | 1,771 | 0,064 | 25,282 | 0,113 | * | FaRIB413 | 8,426 | 0,299 | 3,551 | 0,021 | 8,498 | 0,073 | |
| FaCHC1 | 24,988 | 0,492 | 1,968 | 0,067 | 24,687 | 0,131 | * | FaUBQ1 | 27,222 | 0,566 | 2,079 | 0,039 | 27,322 | 0,081 | |
| FaEF1α | 17,786 | 0,386 | 2,173 | 0,062 | 17,509 | 0,134 | FaCHC1 | 24,163 | 0,427 | 1,767 | 0,129 | 23,712 | 0,228 | ||
| FaGAPDH2 | 19,286 | 0,352 | 1,825 | 0,090 | 18,880 | 0,165 | FaBZIP1 | 25,344 | 0,523 | 2,063 | 0,163 | 25,914 | 0,336 | ||
| FaMT1 | 22,968 | 0,571 | 2,486 | 0,068 | 22,664 | 0,168 | FaEF1α | 16,478 | 0,413 | 2,505 | 0,151 | 15,950 | 0,377 | ||
| FaFHA1 | 23,875 | 0,651 | 2,728 | 0,062 | 24,156 | 0,170 | FaTUBα | 20,364 | 0,539 | 2,649 | 0,236 | 19,538 | 0,625 | ||
| FaTIM1 | 25,885 | 0,813 | 3,139 | 0,116 | 25,363 | 0,364 | FaMT1 | 24,533 | 0,550 | 2,240 | 0,282 | 23,545 | 0,632 | ||
| FaTUBβ | 22,086 | 0,622 | 2,818 | 0,136 | 21,472 | 0,384 | FaFHA1 | 23,116 | 0,612 | 2,646 | 0,246 | 22,256 | 0,650 | ||
| FaTUBα | 20,298 | 0,585 | 2,883 | 0,136 | 19,687 | 0,392 | FaACTIN | 23,145 | 0,776 | 3,352 | 0,343 | 21,943 | 1,151 | ||
| FaACTIN | 24,440 | 0,563 | 2,303 | 0,211 | 23,493 | 0,485 | FaGAPDH1 | 20,908 | 0,836 | 3,999 | 0,401 | 22,313 | 1,605 | ||
| FaBZIP1 | 25,229 | 0,929 | 3,682 | 0,279 | 23,975 | 1,026 | FaTUBβ | 20,592 | 0,964 | 4,681 | 0,436 | 19,067 | 2,040 | ||
| Fungal infection-Camarosa-Petiole (n = 32) | All seven experiments (n = 192) | ||||||||||||||
| * | FaTUBα | 20,767 | 0,423 | 2,036 | 0,007 | 20,737 | 0,014 | * | FaACTIN | 24,011 | 0,883 | 3,676 | 0,004 | 23,905 | 0,015 |
| * | FaACTIN | 23,676 | 0,364 | 1,536 | 0,013 | 23,528 | 0,020 | FaRIB413 | 8,542 | 0,490 | 5,736 | 0,056 | 8,306 | 0,323 | |
| * | FaRIB413 | 8,816 | 0,237 | 2,685 | 0,027 | 8,695 | 0,072 | FaTUBβ | 22,073 | 1,067 | 4,835 | 0,069 | 22,252 | 0,333 | |
| * | FaBZIP1 | 24,874 | 0,620 | 2,493 | 0,034 | 25,025 | 0,084 | FaEF1α | 17,716 | 0,904 | 5,100 | 0,082 | 17,270 | 0,416 | |
| * | FaEF1α | 17,574 | 0,437 | 2,485 | 0,034 | 17,727 | 0,084 | FaMT1 | 24,338 | 1,399 | 5,747 | 0,097 | 24,857 | 0,560 | |
| FaGAPDH2 | 18,321 | 0,357 | 1,946 | 0,064 | 18,611 | 0,125 | FaFHA1 | 24,140 | 1,037 | 4,298 | 0,144 | 23,426 | 0,619 | ||
| FaMT1 | 22,583 | 0,517 | 2,288 | 0,065 | 22,293 | 0,148 | FaTUBα | 21,292 | 1,305 | 6,131 | 0,158 | 21,937 | 0,970 | ||
| FaTUBβ | 22,009 | 0,574 | 2,609 | 0,100 | 21,561 | 0,260 | FaGAPDH1 | 24,722 | 1,856 | 7,509 | 0,157 | 25,119 | 1,175 | ||
| FaCHC1 | 24,874 | 0,735 | 2,954 | 0,139 | 25,499 | 0,411 | FaUBQ1 | 27,492 | 1,134 | 4,124 | 0,295 | 26,149 | 1,217 | ||
| FaUBQ1 | 27,470 | 0,766 | 2,788 | 0,169 | 28,246 | 0,472 | FaGAPDH2 | 18,270 | 1,063 | 5,818 | 0,267 | 17,007 | 1,551 | ||
| FaTIM1 | 25,223 | 0,755 | 2,993 | 0,193 | 26,091 | 0,577 | FaCHC1 | 25,000 | 1,189 | 4,756 | 0,333 | 23,479 | 1,583 | ||
| FaFHA1 | 24,264 | 0,774 | 3,191 | 0,224 | 25,270 | 0,713 | FaBZIP1 | 26,547 | 1,789 | 6,741 | 0,489 | 28,697 | 3,297 | ||
| FaGAPDH1 | 25,419 | 1,120 | 4,405 | 0,263 | 26,600 | 1,156 | FaTIM1 | 25,650 | 1,591 | 6,201 | 0,619 | 22,923 | 3,839 | ||
Genes are ordered into each experiment analyzed, top to bottom, from those tending to show the highest stability to those showing the lowest, based on the stability index. a) "n" represents the number of individuals analyzed from each experiment, (four data points per sample, two biological and two technical replicates of each). b) Data based on analysis of Cq values. SD, standard deviation. CV, Coeficient of variation. c) Slope of regression of gene means. Intercepts are also given for the estimated regression lines. d) Stability index is the product of CV and slope (multiplication of columns 3 and 4). Transcripts with lower slope are preferred as controls. Asterisks mark the best candidate genes with stability index below 0.0×.