Table 2. Behavior data summary for Cx-mGluR5 in C57 background.
| Behavioral paradigm | Measurement | Male | Female | Genotype effect | Gendereffect | Male+Female | Genotype effect | |||
| WT | KO | WT | KO | p value | p value | WT | KO | p value | ||
| Open Field | total distance traveled (cm) | 2716.2±269.3 (16) | 5197.2±423.2 (17) | 4326.0±589.4 (6) | 5000.1±463.6 (7) | 0.002* | 0.139 | 3155.25±291.05 (22) | 5139.75±323.95 (24) | <0.001* |
| movement time (s) | 342.0±21.4 (16) | 379.9±42.1 (17) | 395.2±60.7 (6) | 467.5±49.6 (7) | 0.233 | 0.120 | 356.54±22.43 (22) | 405.29±33.57 (24) | 0.212 | |
| movement speed (cm/s) | 8.68±0.93 (16) | 18.84±4.32 (17) | 11.3±0.3 (6) | 11.0±0.7 (7) | 0.190 | 0.486 | 9.40±0.72 (22) | 16.55±3.13 (24) | 0.038* | |
| vertical activity | 186.6±29.8 (16) | 223.5±23.9 (17) | 212.2±57.4 (6) | 200.6±52.1 (7) | 0.745 | 0.001* | 193.57±26.10 (22) | 216.79±22.19 (24) | 0.499 | |
| center zone distance (cm) | 623.4±105.4 (16) | 1069.5±154.1 (17) | 941.2±414.4 (6) | 1156.9±240.7 (7) | 0.193 | 0.973 | 710.10±133.68 (22) | 1094.94±127.13 (24) | 0.043* | |
| center zone duration (s) | 227.8±37.5 (16) | 296.6±36.4 (17) | 235.2±129.3 (6) | 327.0±75.5 (7) | 0.117 | 0.333 | 229.83±42.64 (22) | 305.48±33.05 (24) | 0.164 | |
| center distance ratio | 0.20±0.03 (16) | 0.21±0.03 (17) | 0.17±0.06 (6) | 0.24±0.04 (7) | 0.315 | 0.758 | 0.19±0.02 (22) | 0.22±0.02 (24) | 0.417 | |
| Elevated Plus Maze | time spent in open arm (s) | 146.7±29.0 (10) | 160.8±20.5 (9) | 211.1±34.6 (4) | 208.3±17.1 (4) | 0.857 | 0.084 | 165.07±23.68 (14) | 175.40±16.01 (13) | 0.654 |
| number of open arm entries | 16.0±1.6 (10) | 20.0±2.5 (9) | 16.3±1.3 (4) | 25.3±3.1 (4) | 0.016* | 0.281 | 16.07±1.17 (14) | 21.62±2.00 (13) | 0.275 | |
| number of head dips | 24.9±3.7 (10) | 20.7±4.6 (9) | 12.8±5.6 (4) | 26.8±3.8 (4) | 0.346 | 0.556 | 21.43±3.35 (14) | 22.54±3.41 (13) | 0.854 | |
| number of rearings | 47.3±5.6 (10) | 37.6±5.1 (9) | 23.5±6.1 (4) | 53.5±11.2 (4) | 0.170 | 0.588 | 40.5±5.2 (14) | 42.5±5.1 (13) | 0.790 | |
| number of groomings | 8.5±1.3 (10) | 6.4±1.4 (9) | 9.5±1.7 (4) | 10.5±3.2 (4) | 0.781 | 0.191 | 8.79±1.04 (14) | 7.69±1.40 (13) | 0.568 | |
| distance traveled in close arm (m) | 8.3±0.6 (10) | 6.8±0.6 (9) | 7.4±0.7 (4) | 9.7±0.5 (4) | 0.554 | 0.167 | 8.0±0.5 (14) | 7.7±0.6 (13) | 0.680 | |
| 3 Chamber | preference ratio - object (habituation) | 1.39±0.15 (21) | 1.16±0.19 (20) | n.a. | n.a. | 0.341 | n.a. | n.a. | n.a. | n.a. |
| preference ratio - chamber (habituation) | 1.19±0.15 (21) | 1.11±0.12 (20) | n.a. | n.a. | 0.678 | n.a. | n.a. | n.a. | n.a. | |
| preference ratio - object (social) | 2.57±0.39 (21) | 2.82±0.45 (20) | n.a. | n.a. | 0.677 | n.a. | n.a. | n.a. | n.a. | |
| preference ratio - chamber (social) | 1.98±0.36 (21) | 1.47±0.21 (20) | n.a. | n.a. | 0.240 | n.a. | n.a. | n.a. | n.a. | |
| Conditioned Fear | context (% time freezing) | 10.9±2.5 (9) | 21.1±6.9 (9) | 16.0±3.9 (8) | 16.9±6.6 (8) | 0.152 | 0.933 | 13.32±2.26 (17) | 19.11±4.68 (17) | 0.274 |
| CS – uncued (% time freezing) | 2.90±1.10 (9) | 12.17±4.63 (9) | 3.00±1.01 (8) | 2.21±0.74 (8) | 0.114 | 0.067 | 2.94±0.73 (17) | 7.48±2.71 (17) | 0.116 | |
| CS – cued (% time freezing) | 66.93±3.10 (9) | 70.23±3.27 (9) | 65.52±6.82 (8) | 55.83±5.52 (8) | 0.508 | 0.114 | 66.26±3.49 (17) | 63.45±3.51 (17) | 0.574 | |
| Light-Dark | dark-duration (s) | 478.70±25.04 (10) | 455.24±30.89 (14) | 457.56±21.63 (10) | 400.21±28.31 (11) | 0.160 | 0.184 | 468.13±16.29 (20) | 431.03±21.62 (25) | 0.196 |
| dark-entry count | 25.20±5.33 (10) | 25.64±4.37 (14) | 39.0±6.6 (10) | 46.7±6.4 (11) | 0.476 | 0.004* | 32.10±4.43 (20) | 34.92±4.24 (25) | 0.651 | |
| dark-latency (s) | 68.6±36.9 (10) | 18.4±10.7 (14) | 39.5±17.8 (10) | 34.1±17.0 (11) | 0.197 | 0.297 | 54.05±20.23 (20) | 25.28±9.52 (25) | 0.177 | |
| light-duration (s) | 118.3±23.7 (10) | 143.6±31.0 (14) | 142.3±21.6 (10) | 199.6±28.3 (11) | 0.148 | 0.571 | 130.33±15.85 (20) | 168.26±21.68 (25) | 0.184 | |
| light-entry count | 29.9±6.4 (10) | 26.8±4.4 (14) | 39.2±6.6 (10) | 46.8±6.4 (11) | 0.703 | 0.366 | 34.55±4.58 (20) | 35.60±4.19 (25) | 0.867 | |
| Footslip Assay | number of footfalls | 307.9±29.3 (10) | 372.3±29.9 (8) | n.a. | n.a. | 0.148 | n.a. | n.a. | n.a. | n.a. |
| footfalls: time active (s) | 70.1±9.9 (10) | 80.5±13.7 (8) | n.a. | n.a. | 0.535 | n.a. | n.a. | n.a. | n.a. | |
| footfalls: mean active duration (s) | 0.2±0.0 (10) | 0.2±0.1 (8) | n.a. | n.a. | 0.687 | n.a. | n.a. | n.a. | n.a. | |
| footfalls: activation frequency | 0.5±0.1 (10) | 0.6±0.1 (8) | n.a. | n.a. | 0.149 | n.a. | n.a. | n.a. | n.a. | |
| distance traveled (m) | 10.4±1.0 (10) | 15.4±2.1 (8) | n.a. | n.a. | 0.031* | n.a. | n.a. | n.a. | n.a. | |
| Prepulse Inhibition | % PPI at 74 dB prepulse | 30.7±5.0 (8) | 18.8±5.8 (8) | 20.5±10.3 (9) | 9.3±7.4 (9) | 0.142 | 0.208 | 25.30±5.91 (17) | 13.78±4.77 (17) | 0.139 |
| % PPI at 78 dB prepulse | 35.8±9.1 (8) | 41.4±10.1 (8) | 31.4±10.7 (9) | 26.6±9.1 (9) | 0.967 | 0.330 | 33.45±6.89 (17) | 33.55±6.81 (17) | 0.991 | |
| % PPI at 82 dB prepulse | 48.6±6.9 (8) | 50.4±10.0 (8) | 52.9±7.6 (9) | 45.4±11.3 (9) | 0.758 | 0.967 | 50.86±5.05 (17) | 47.73±7.38 (17) | 0.729 | |
| normalized startle response (A.U./g body weight) | 1099.9±228.8 (8) | 846.1±131.0 (8) | 482.9±76.5 (9) | 598.5±121.5 (9) | 0.638 | 0.006* | 773.23±135.09 (17) | 715.02±91.64 (17) | 0.724 | |
| Marble Burying | marbles buried | 11.5±1.4 (11) | 7.6±1.0 (14) | 7.1±1.1 (11) | 7.0±1.3 (12) | 0.109 | 0.041* | 9.27±0.99 (22) | 7.35±0.778 (26) | 0.129 |
Values are listed as mean ± SEM (animals number). Abbreviations: A.U., arbitrary unit;
*
, p<0.05.