Fig. 7.

Effects of Mg²⁺ and Ca²⁺ on DEA/NO decomposition and GSNO formation. Shown are the average peak heights (n=5) observed immediately after DEA/NO addition in the absence and presence of GSH, as well as the peak height after CuSO₄ addition in the presence of GSH with and without 5 mM MgCl₂ or CaCl₂. Experimental conditions: 1 µM DEA/NO, 2 mM GSH as indicated, 4 mM CuSO₄, 1000 U/ml SOD, 0.1 mM DTPA, and 50 mM TEA (pH 7.4) in 0.5 ml at 37 °C.